New polymorphic microsatellite loci for the Macedonian crested newt, Triturus macedonicus, and cross-priming testing in four other crested newt species
Submitted by Panagiotis Kasapidis on Sun, 2011-03-27 20:39
Title | New polymorphic microsatellite loci for the Macedonian crested newt, Triturus macedonicus, and cross-priming testing in four other crested newt species |
Publication Type | Journal Article |
Year of Publication | 2008 |
Authors | Sotiropoulos, K, Tsaparis D, Eleftherakos K, Kotoulas G, Legakis A, Kasapidis P |
Journal | Molecular Ecology Resources |
Volume | 8 |
Pages | 1402-1404 |
Keywords | conservation, crested newt, microsatellites, population genetics, Triturus macedonicus |
Abstract | Eleven microsatellites have been characterized for Triturus macedonicus. Nine loci showed different variation patterns in a sample of 40 individuals from a single breeding pond in Zagori province (Greece), with an average number of 4.2 alleles per locus and an expected heterozygosity ranging from 0.1199 to 0.8079. Distinct cross-priming amplification rates were recovered on four additional crested newt species. Two monomorphic T. macedonicus loci were polymorphic in other Triturus species. The microsatellites developed herein could be a useful intraspecific genetic tool to undertake fine-scale population genetic analyses as well as in the study of contact zones between crested newt species. |
URL | www.scopus.com |
DOI | 10.1111/j.1755-0998.2008.02318.x |